Since 1893, students from around the world have congregated at the Marine Biological Laboratory for the popular Embryology Advanced Research Training Course. The students in this year’s course, Embryology Concepts & Techniques in Modern Developmental Biology, have produced some eye-catching images during their time in Woods Hole.
This image of the arthropod Springtail (Collembola) was captured on a Flamingo T-SPIM lightsheet microscope. Credit: Guilherme (Gui) Gainett and Carsten Wolff.
Male nematode (C. Elegans) spicules imaged on Zeiss Micro 900 with GFP laminin. Credit: Rebecca M Varney, Postdoc, UCSB.
Egg chamber of fruit fly (Drosophila) imaged on a Zeiss LSM 900. Nuclei (cyan), F-actin (grey), polar cells (orange). Credit: Louis Prahl, postdoc, University of Pennsylvania
A transgenic quail from Peter Lwigale‘s lab, it is expressing fluorescence (GFP) to target vasculature, which you can see quite well around the eye at this stage. Imaged on a Leica Micro Thunder. Credit: Evan Curcio
Fruit Fly (Drosophila) egg chamber. Actin filaments in magenta and nucleus/DNA in grey. Taken during #Embryo21 on ZEISS microscope. Credit: Viraj Doddihal, Graduate Student at the Stowers Institute for Medical Research, under direction of Sally Horne-Badovinac
Early stage fruit fly (drosophila) embryos captured on a Nikon Ti2 microscope with a Yokogawa W1 spinning disk confocal. The colors are krüppel (green) and knirps (magenta) in situ hybridizations. Staining done by scientists in the Nipam Patel Lab. Credit: Louis Prahl, postdoc, University of Pennsylvania
A set of lab gap genes in the amphipod crustacean (Parhyale hawaiensis). Genes expressed: dll-e (green), hth (purple), dac2 (blue), sp6-9 (orange), DIC (grayscale), Bottom right is a merged image showing all other at once. Credit: Brittany Truong, PhD Candidate, University of Colorado
An embryo of the fruit fly (drosophila) imaged under a Zeiss 780 Confocal Microscope. Blue is DAPI for nuclei; red is motor neurons, green is muscle, and pink is central nervous system. Credit: Rebecca M Varney, Postdoc, UCSB.
For more microscopy images, follow #Embryo21 on Twitter.
The students in the Embryology course also made it off the lab bench and into the sunshine for a trip on the MBL’s Collection Vessel The Gemma.